Jo, I had to collect urine sampled to do drug test when I worked in the deliquent boy's grouphome. They tried all kinds of things to get the tests to read normal. I had the best success with "first pee of the morning" samples after they slept all night. You might want to do this with your home pregnancy test. The first pee urine sample seems to be more concentrated. My daughter had undeterminable results with one of her pregnancy test until she did the first thing in the morning. Then the test was easy to read.