If you did take it off, why would you be doing it? If it is to get a reaction from him then I would say no. Are you trying to shock him into R, are you doing it out of anger at him or are you doing it because you think your marriage is over.
I think I told you I took my ring off first the day after he MO?. He came by in the morning after going to the gym to take me to the station. I noticed he had taken his wedding ring off. I threw mine at him. Turned out he just hasn’t put it back on after his work out (he did this sometimes). He never put it back on. I regret taking mine off to this day.
