are you & your wife still physically intimate with each other?
Woman don't always like to initiate the physical part (truth be told I bet you it's the majority of women that want their men to take the initiative/lead instead of asking if it's ok, some women want to be "taken", it shows how secure you are with yourself.)
Regardless of rejection, even if she had said no possibly if you had made your advances to her, in the end, not making the attempt at all guaranteed that you rejected yourself first before she even had a chance. If this isn't clear let me know and I'll explain further.
Next time you get in this situation and you want to be sexual with your wife, don't let the fear of her rejection stop you from trying altogether - when you do that, you guarantee 100% that nothing will happen.
Rejection may suck but sometimes I think guys shoot ourselves in the foot but assuming that rejection is a given, it's not always the case and you end up missing out on an opportunity to be close to your wife.